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Laser ablation electrospray ionization : ウィキペディア英語版 | Laser ablation electrospray ionization Laser Ablation Electrospray Ionization (LAESI) is an ambient ionization method for mass spectrometry that combines laser ablation from a mid-infrared (mid-IR) laser with a secondary electrospray ionization (ESI) process. The mid-IR laser is used to generate gas phase particles which are then ionized through interactions with charged droplets from the ESI source. LAESI was developed by Professor Akos Vertes and Dr. Peter Nemes in 2007 and is now marketed commercially by Protea Biosciences, Inc. LAESI is a novel ionization source for mass spectrometry (MS) that has been used to perform MS imaging of plants,〔〔〔 tissues,〔〔〔〔 cell pellets,〔 and even single cells.〔〔〔〔 In addition, LAESI has been used to analyze historic documents〔 and untreated biofluids such as urine and blood.〔 The technique of LAESI is performed at atmospheric pressure and therefore overcomes many of the obstacles of traditional MS techniques, including extensive and invasive sample preparation steps and the use of high vacuum.
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